Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody
(1 reference)-
- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG (H&L)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Anti-Mouse IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Mouse IgG whole molecule
- Isotype
- IgG
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Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.
ELISA Dilution: 1:20,000 - 1:400,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Comment
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Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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: "Immune Monitoring of Trans-endothelial Transport by Kidney-Resident Macrophages." in: Cell, Vol. 166, Issue 4, pp. 991-1003, (2016) (PubMed).
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: "Immune Monitoring of Trans-endothelial Transport by Kidney-Resident Macrophages." in: Cell, Vol. 166, Issue 4, pp. 991-1003, (2016) (PubMed).
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: rabbit anti-Mouse IgG Antibody, rabbit anti-mouse IgG heavy and light chain antibody, rb anti-ms IgG heavy and light chain, Anti-Mouse Secondary Antibody
Background: Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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