14-3-3 GRF antibody

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Antigen
Reactivity
Arabidopsis thaliana, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Options
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Immunogen conserved surface-exposed peptide conjugated to KLH.This sequence is repeated in all 12 -canonical- members of the Arabidopsis thaliana GRF/14-3-3 family. GRF1 GF14 chi UniProt: F4JJ94, TAIR: AT4G09000,GRF2 GF14 omega, UniProt:Q01525, TAIR:AT1G78300, GRF3 GF14 psi UniProt: F4KBI7 , TAIR:AT5G38480,GRF4 GF14 phi UniProt:F4HWQ5, TAIR: AT1G35160,GRF5 GF14 upsilon UniProt: P42645, TAIR:AT5G16050,GRF6 GF14 lambda UniProt: P48349#P48349-2, TAIR:AT5G10450,GRF7 GF14 nu UniProt:Q96300, TAIR:AT3G02520,GRF8 GF14 kappa UniProt:F4KHY7, TAIR:AT5G65430,GRF9 GF14 mu UniProt: F4IP55, TAIR:AT2G42590, GRF10 GF14 epsilon UniProt: F4I1C1, TAIR:AT1G22300, GRF11 GF14 omicron UniProt: Q9S9Z8, TAIR: AT1G34760, GRF12 GF14 iota UniProt:Q9C5W6 TAIR: AT1G26480.
Specificity TAIR: At4g09000, At4g78300, At4g38480, At4g35160, At4g16050, At4g10450, At4g02520, At4g65430, At4g42590, At4g22300, At4g34760
Cross-Reactivity (Details) Not reactive in: This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum.
Predicted Reactivity Triticum aestivum, Zea mays, Oryza sativa, Pisum sativum, Nicotiana tabacum, Solanum tuberosum, Gossypium hirsutum, Brassica sp., Physcomitrella patens, Chlamydomonas reinhardtii
Characteristics Expected / apparent Molecular Weight of the Antigene: 28-20 kDa (depending upon an isoform)
Purification serum
Antigen
Background GRF (General regulatory factor 2) or 14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway.
UniProt F4JJ94, Q01525, F4KBI7, F4HWQ5, P42645, Q96300, F4KHY7, F4IP55, F4I1C1, Q9S9Z8, Q9C5W6
Application Notes 1: 2000 (WB)
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution please add 50 μL of sterile water
Storage Comment Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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