Apo-B100 ELISA Kit
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- Target See all Apo-B100 ELISA Kits
- Apo-B100 (Apoprotein B100 (Apo-B100))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 2.5-600 ng/mL
- Minimum Detection Limit
- 2.5 ng/mL
- Application
- ELISA
- Purpose
- Human ApoB100 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair detects human ApoB100. Other species not determined.
- Sensitivity
- 2.5 ng/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Apoprotein B100 (Apo-B100) ELISA Kit
Apo-B100 Reactivity: Human Colorimetric Sandwich ELISA 23.44 ng/mL - 1500 ng/mL Plasma, Serum
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Human Colorimetric Competition ELISA 78 ng/mL - 5000 ng/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Urine
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Rat Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Rat Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Saliva, Serum, Urine
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Mouse Colorimetric Sandwich ELISA 78.13 ng/mL - 5000 ng/mL Plasma, Saliva, Serum, Urine
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Human Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Saliva, Serum, Urine
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Human Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apoprotein B100 (Apo-B100) ELISA KitApo-B100 Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
Apoprotein B100 (Apo-B100) ELISA KitWide Range Apo-B100 Reactivity: Rat Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Application Notes
- Recommended Dilution for serum and plasma samples10,000 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Sample Preparation
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For example, add 2 μL of serum or plasma into a tube with 98 μL 1X Assay Diluent to prepare a 50-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 50-fold diluted sample into a tube with 398 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all Apo-B100 ELISA Kits
- Apo-B100 (Apoprotein B100 (Apo-B100))
- Alternative Name
- ApoB100 (Apo-B100 Products)
- Synonyms
- Ac1-060 ELISA Kit, Apo B-100 ELISA Kit, ApoB-100 ELISA Kit, ApoB-48 ELISA Kit, apolipoprotein B ELISA Kit, Apob ELISA Kit
- Background
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Gene Names: APOB
Protein names: Apolipoprotein B-100 (Apo B-100) [Cleaved into: Apolipoprotein B-48 (Apo B-48)]
- Gene ID
- 338
- UniProt
- P04114
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