Apoprotein B100 (apo-B100) ELISA Kit

Details for Product No. ABIN2702829, Supplier: Log in to see
Antigen
  • Ac1-060
  • Apo B-100
  • ApoB-100
  • ApoB-48
  • apolipoprotein B
  • Apob
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
20
15
14
8
6
5
3
3
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
2.5-600 ng/mL
Minimum Detection Limit
2.5 ng/mL
Application
ELISA
Options
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Supplier Product No.
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Purpose Human ApoB100 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Cell Culture Supernatant, Plasma, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair detects human ApoB100. Other species not determined.
Sensitivity 2.5 ng/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Antigen
Alternative Name ApoB100
Background Gene Names: APOB
Protein names: Apolipoprotein B-100 (Apo B-100) [Cleaved into: Apolipoprotein B-48 (Apo B-48)]
Gene ID 338
UniProt P04114
Application Notes Recommended Dilution for serum and plasma samples10,000 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Sample Preparation

For example, add 2 μL of serum or plasma into a tube with 98 μL 1X Assay Diluent to prepare a 50-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 50-fold diluted sample into a tube with 398 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample.

Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
ELISA image for Apoprotein B100 (apo-B100) ELISA Kit (ABIN2702829) Apoprotein B100 (apo-B100) ELISA Kit
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