Use your antibodies-online credentials, if available.
No Products on your Comparison List.
Your basket is empty.
Find out more
Get this product for free
The calibration curve concentrations used for the ELISA's were 1000 pg/mL, 333.33 pg/mL, 111.11 pg/mL, 37.04 pg/mL, 12.35 pg/mL.
This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between CT concentration in the sample and the assay signal intensity. Average the duplicate readings for each calibrator, control, and samples. Create a calibration curve on log-log or semi-log graph paper, with the log of CT concentration on the y-axis and absorbance on the x- axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.