The GFP-Trap® is a monovalent matrix (agarose or magnetic beads) with GFP-binding proteins coupled to it. GFP-Traps® are ideal for efficient, one-step isolation of fluorescent fusion proteins and their interacting partners (look at the overview diagram below).
Fluorescent proteins (FPs) are useful tools for detecting events in living cells and animals. The discovery of the green fluorescent proteins (GFP) in the early 1960s has revolutionized cell biology by enabling scientists to track a wide variety of proteins and enzyme targets in vivo. Fluorescent proteins (FP) can be used as a fluorescent label to track gene expression, nuclear localization and dynamics in cells.
Such in vivo data, however, should ideally be combined with biochemical methods (in vitro) to compliment the cell biological methods. This naturally calls for a reliable and efficient tool, such as the GFP-Trap®, to enable the execution of such biochemical experiments.
The GFP-Traps® are based on antibodies from the Camelidae (camels, dromedaries, llamas and alpacas) family. Camalidae antibodies bind to their antigens via the VHH domains. The VHH domains are small (~13 kDa), chemically stable and have high specificity and high affinities to their antigens. In short, these desirable characteristics make Camelidae antibodies ideal tools for downstream applications using biochemical methods.
- Pulldowns & Immunoprecipitations
- Mass spectroscopy
- Enyzme activity measurements
Immunoprecipitations (IP) of GFP from protein extracts of human cells. Input (I), flow through (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western Blotting. In comparison, IP with GFT-Traps shows no contaminating heavy chain (hc) and light chain (lc) of conventional antibodies. In addition to the GFP®-Trap, there is also the RFP-Trap® available (coupled to agarose or magnetic beads).
You may find a manual and additional publications for both variants on our website.
Need a binding control to check for unspecific binding? Or do you want to deplete unspecifically binding proteins prior to the pulldown?
- Blocked agarose beads
- Blocked magnetic beads
Pulldown of different monomeric red fluorescent proteins, mRFP, mCherry and mOrange from cell extracts of human cells. Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining.