SARS-CoV-2 Spike Protein (P.1 - gamma) (rho-1D4 tag)
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- Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern P.1.
- Contains L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, T1027I, and V1176F mutations characteristic for the SARS-CoV-2 gamma variant (P.1).
- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Protein Characteristics
- P.1 - gamma
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Origin
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.
- Purpose
- This is the spike protein of the mutant strain P.1, also commonly known as the "Brazil mutant".
- Specificity
- Mutation that differ from canonical sequence of SPIKE protein: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, T1027I, V1176F
- Characteristics
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"SARS CoV-2 full-length Spike P.1 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent. - Purification
- affinity chromatography
- Purity
- > 98 % as determined by SDS-PAGE
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha Host: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa Host: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
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- Comment
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Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker) - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Avoid freeze-thaw cycles
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: "Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." in: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).
: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).
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: "Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." in: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).
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- Target
- SARS-CoV-2 Spike
- Alternative Name
- SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Molecular Weight
- 142114 Da
- UniProt
- P0DTC2
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