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CUT&RUN Antibodies

CUT&RUN overcomes various downfalls of ChIP-Seq with improved workflow. CUT&RUN is simple to perform and is inherently robust. Our CUT&RUN antibodies against histone post-translational modifications and other targets have been tested very thoroughly to ensure highest reliability and best performance.

To identify best-in-class antibodies for use in CUT&RUN assays, antibodies-online has developed a rigorous validation process working with customers and manufacturers. This allows us to provide you with an ever-increasing portfolio of reliable antibodies for this innovative method. Therefore, it is best to check our websites regularly.

Tips for Antibody Selection

The successful execution of cleavage under target and release using nuclease (CUT&RUN) hinges on antibody selection. A factor- or histone-specific antibody is bound to chromatin in situ followed by binding to the antibody of a protein A-micrococcal nuclease (pA-MNase) fusion. As is the case with ChIP, the success depends in large part on the affinity of the antibody for its target and its specificity under the conditions used for binding. We compiled a list of primary and secondary antibodies suitable for CUT&RUN down below. Addtionally, antibodies-online supports the validation of antibodies for CUT&RUN. You can participate in our independent validation initiative (IVI). Propose and perform a validation experiment and get a full refund on the validated antibody. Please contact us, if you are interested in validating one of our CUT&RUN products!

Antibodies validated for CUT&RUN Assays

Histone Modification

Product
Source
Clonality
Application
Cat. No.
Validations
Quantity
Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN
Cat. No. ABIN6971977
Validations
  • (4)
  • (1)
Quantity 100 μg
Datasheet Datasheet
Source Mouse
Clonality Monoclonal
Application WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag
Cat. No. ABIN2668403
Validations
  • (4)
  • (5)
  • (1)
Quantity 100 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag
Cat. No. ABIN2668472
Validations
  • (75)
  • (5)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag
Cat. No. ABIN3023254
Validations
  • (5)
  • (10)
Quantity 100 μL
Datasheet Datasheet
Source
Clonality
Application CUT&RUN, CUT&Tag
Cat. No. ABIN6923144
Validations
  • (1)
  • (1)
Quantity 135 μL
Datasheet Datasheet
Source Mouse
Clonality Monoclonal
Application IF, FACS, IHC, StM, CUT&RUN
Cat. No. ABIN6939594
Validations
  • (8)
Quantity 100 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN
Cat. No. ABIN3434046
Validations
  • (5)
  • (5)
Quantity 100 μL
Datasheet Datasheet
Source Mouse
Clonality Monoclonal
Application WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag
Cat. No. ABIN2668392
Validations
  • (2)
  • (5)
Quantity 100 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN
Cat. No. ABIN6971937
Validations
  • (4)
Quantity 100 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag
Cat. No. ABIN2667903
Validations
  • (1)
Quantity 10 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, SNAP-ChIP
Cat. No. ABIN6952339
Validations
  • (1)
Quantity 50 μg
Datasheet Datasheet
Source Rabbit
Clonality Monoclonal
Application WB, ELISA, ChIP, ICC, MA, CUT&RUN
Cat. No. ABIN2830942
Validations
Quantity 100 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag, PeA
Cat. No. ABIN6952337
Validations
Quantity 50 μg
Datasheet Datasheet

Transcription Regulator

Product
Source
Clonality
Application
Cat. No.
Validations
Quantity
Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, FACS, IHC (p), ICC, IP, IHC (fro), CUT&RUN
Cat. No. ABIN2855074
Validations
  • (11)
  • (15)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, IP, IHC (p), ICC, ChIP
Cat. No. ABIN2854987
Validations
  • (1)
  • (9)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, IHC (p), ICC
Cat. No. ABIN2855865
Validations
  • (1)
  • (8)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IHC, IF, IHC (p), FACS, IP, ICC, IHC (fro), ChIP, IHC (wm), CUT&RUN
Cat. No. ABIN2855042
Validations
  • (8)
  • (14)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, ChIP-seq, ChIP, CUT&RUN
Cat. No. ABIN6972849
Validations
  • (1)
  • (4)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB
Cat. No. ABIN1680678
Validations
  • (1)
  • (6)
  • (1)
Quantity 100 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IHC, IF
Cat. No. ABIN6140332
Validations
  • (8)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, ELISA, ICC, IF, CUT&RUN
Cat. No. ABIN6265491
Validations
  • (3)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Monoclonal
Application WB, IHC, IF (cc), IHC (p), FACS
Cat. No. ABIN6945139
Validations
  • (1)
  • (2)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Monoclonal
Application WB, IHC (p), IF (cc)
Cat. No. ABIN5557532
Validations
  • (3)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, ELISA, IHC (p), IF (p), FACS
Cat. No. ABIN1387620
Validations
  • (2)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IP, CUT&RUN
Cat. No. ABIN6972727
Validations
  • (2)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC
Cat. No. ABIN1386749
Validations
  • (1)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application IF (cc), IF (p), IHC (p), IHC (fro), ICC
Cat. No. ABIN1714202
Validations
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ICC, CUT&RUN, ChIP-seq, ChIP
Cat. No. ABIN6972778
Validations
  • (1)
  • (3)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ICC, ChIP-seq, ChIP, CUT&RUN
Cat. No. ABIN6972403
Validations
  • (3)
Quantity 100 μL
Datasheet Datasheet

Chromatin Remodeler

Product
Source
Clonality
Application
Cat. No.
Validations
Quantity
Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, IHC (p), IP, ICC, ChIP, IHC (fro), IHC (wm)
Cat. No. ABIN2854776
Validations
  • (7)
  • (20)
  • (1)
Quantity 100 μL
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, ELISA, IF, IHC (p), CUT&RUN
Cat. No. ABIN6991990
Validations
  • (2)
  • (1)
Quantity 0.1 mg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, ChIP-seq, ChIP, CUT&RUN
Cat. No. ABIN6971459
Validations
  • (3)
Quantity 100 μL
Datasheet Datasheet

NA Metabolism

Product
Source
Clonality
Application
Cat. No.
Validations
Quantity
Datasheet
Source Mouse
Clonality Monoclonal
Application WB, ELISA, ChIP, FM, CUT&RUN
Cat. No. ABIN6655367
Validations
  • (7)
Quantity 50 μg
Datasheet Datasheet
Source Mouse
Clonality Monoclonal
Application WB, ELISA, ChIP, FM, CUT&RUN
Cat. No. ABIN6655366
Validations
  • (7)
Quantity 50 μg
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Application WB, IF, ICC, IHC (p), CUT&RUN
Cat. No. ABIN2856044
Validations
  • (3)
  • (3)
Quantity 100 μL
Datasheet Datasheet

Controls for CUT&RUN

Our secondary antibody ABIN101961 was used as negative control and is referenced in the original paper on the CUT&RUN method (Peter J. Skene and Steven Henikoff, 2018). Use it in your CUT&RUN assays as well:

Product
Source
Clonality
Binding Specificity
Cat. No.
Delivery
Validations
Datasheet
Source Guinea Pig
Clonality Polyclonal
Binding Specificity Heavy & Light Chain
Cat. No. ABIN101961
Delivery 1 to 2 Days
Validations
  • (105)
  • (3)
  • (1)
Datasheet Datasheet
Source Rabbit
Clonality Polyclonal
Binding Specificity Heavy & Light Chain
Cat. No. ABIN101785
Delivery 1 to 2 Days
Validations
  • (4)
  • (1)
Datasheet Datasheet

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