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CUT&RUN

CUT&RUN stands for cleavage under targets and release using nuclease. The technique developed from the Henikoff Lab offers a new approach to pursue epigenetics. CUT&RUN introduces some major modifications to eliminate shortcomings inherent to ChIP-seq. It is simple to perform and inherently robust, with extremely low backgrounds requiring only ~1/10th the sequencing depth as ChIP. CUT&RUN is cost-effective for transcription factor and chromatin profiling.

Download Now: Epigenome Profiling with CUT&RUN and CUT&Tag Poster

CUT&RUN Antibodies

A range of antibodies validated for use in CUT&RUN assays.

CUT&RUN Sets

CUT&RUN made easy - with our sets & components

CUT&RUN ConA Beads

Magnetic ConA Beads for usage in CUT&RUN assays

CUT&RUN Webinar antibodies-online

CUT&RUN - Better than ChIP-seq!

Better Data
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Only 1/3 of seq reads required
Less Signal Noise
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Easer Peak-Calling, Higher Reproducibility
Less Sample
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10x less sample in comparison to ChIP-seq
Optimized Protocol
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Obtain purified DNA from Cells within 1 day

Get started with CUT&RUN

antibodies-online offers a comprehensive range of products for CUT&RUN: CUT&RUN sets, antibodies validated for CUT&RUN, pAG-MNase, magnetic Concanavalin A beads, positive and negative control antibodies and more. Our secondary antibody ABIN101961 was used in different original papers for CUT&RUN by S. Henikoff. Discover our product range.

CUT&RUN Antibodies

A range of antibodies validated for use in CUT&RUN assays.

CUT&RUN Sets

CUT&RUN made easy - with our sets & components

CUT&RUN ConA Beads

Magnetic ConA Beads for usage in CUT&RUN assays

Get our free CUT&RUN and CUT&Tag handbook now!

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Why choose CUT&RUN Products from antibodies-online?

We at antibodies-online became aware of the great benefits of CUT&RUN technology very early on and have been paying particularly close attention to it. We are working intensively on products that make this innovative method even more efficient and easier. We regularly make our findings available in our own CUT&RUN protocol as well as in webinars and brochures.

Our CUT&RUN antibodies, sets and components offer you the highest quality and reliability. If you have any questions, please do not hesitate to contact our CUT&RUN expert PhD Stefan Pellenz.

  • Extensively validated products
  • Components precisely matched to each other
  • More and more antibodies validated for CUT&RUN
  • Scientific support for questions and problems

Importance of antibody selection

IVI Logo

antibodies-online supports the validation of antibodies for CUT&RUN. Together with customers and manufacturers we are currently running more than 50 validation projects. And new ones are added almost weekly. You can participate in our independent validation initiative (IVI). Propose and perform a validation experiment (CUT&RUN) and receive full reimbursement for the validated antibody. Contact us via email without any obligation!

Targets referenced in CUT&RUN / CUT&Tag Publications

Based on published work, the majority of antigens for a targeted fragmentation of genomic DNA by CUT&RUN and CUT&Tag are histone modifications such as repressive H3K27me3 or H3K4me3 associated with active promoters. Other protein targets are transcription regulators like transcription factors CTCF or SALL4, chromatin modifiers like HDAC2 or proteins involved in nucleic acid metabolism such as Pol II. Most of the works were done with samples of human or murine origin. For a number of these targets, we already offer antibodies explicitly validated for use in CUT&RUN assays.

Number of publications mentioning the indicated antigens in context with CUT&RUN and CUT&Tag based on 45 journal articles published from 2018 to 2021.

Fig. 1: Number of publications mentioning the indicated antigens in context with CUT&RUN and CUT&Tag based on 45 journal articles published from 2018 to 2021.

Download Additional CUT&RUN Resources

Additional CUT&RUN Resources


References

  1. Skene, Henikoff: "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites." in: eLife, Vol. 6, (2018) (PubMed).
  2. Brahma, Henikoff: "RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast." in: Molecular cell, Vol. 73, Issue 2, pp. 238-249.e3, (2019) (PubMed).
  3. Meers, Bryson, Henikoff, Henikoff: "Improved CUT&RUN chromatin profiling tools." in: eLife, Vol. 8, (2019) (PubMed).
  4. Henikoff, Henikoff, Kaya-Okur, Ahmad: "Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation." in: eLife, Vol. 9, (2020) (PubMed).
Julian Pampel
Julian Pampel, BSc
Content Manager at antibodies-online.com

Creative mind of antibodies-online with a keen eye for details. Proficient in the field of life-science with a passion for plant biotechnology and clinical study design. Responsible for illustrated and written content at antibodies-online as well as supervision of the antibodies-online scholarship program.

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